Figure 1.

Expression and transcriptional modulation of HLA-II in macrophages infected by H. pylori. (A) Surface expression of HLA-II protein in human macrophages infected by H. pylori (MOI 10) for 6, 24, 48, and 72 h. Data are expressed as median fluorescence intensity (MFI) ± SEM of five independent experiments. (B) HLA-II total cell content evaluated by Western blot in macrophages infected as in (A). Blot refers to a representative of three independent experiments. (C) Relative expression of mRNA encoding CIITA in macrophages infected as in (A). Data were normalized to an endogenous reference gene (β-actin). Values at T₀ cells were taken as reference and set as 1 and the expression levels for treated cells were relative to the expression of T₀ cells. Data are expressed as mean ± SEM of five independent experiments. Significance was determined by Student's t-test. *p < 0.05; **p < 0.01; and ***p < 0.001.