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Catalytic efficiency of wild-type UGT76C5 and mutants. Kinetic parameters for UDP-glucose were determined with 1.0 mM NA.
| Enzyme | Km (mM) | Kcat (x 10-3, s-1) | Kcat/Km (s-1M-1) |
|---|---|---|---|
| UGT76C5 (WT) | 0.33 ± 0.022 | 0.89 ± 0.053 | 2.69 ± 0.11 |
| 76C5② | 0.17 ± 0.008 | 2.04 ± 0.053 | 12.0 ± 0.26 |
| 76C5①② | 0.20 ± 0.007 | 1.55 ± .072 | 7.70 ± 0.17 |
| 76C5②③ | 0.19 ± 0.019 | 1.56 ± 0.11 | 8.36 ± 0.31 |
| 76C5①②③ | 0.21 ± 0.009 | 1.49 ± 0.033 | 6.93 ± 0.17 |
