Table 2.

Voltage-dependent inactivation (VDI) during a 5-s depolarizing pulse from a HP of − 89 mV to V_max was quantified by calculating the residual Ba²⁺ current fraction after 250, 1000, or 5000 ms (r250, r1000, r5000)

Construct	r250 [%]	r500 [%]	r1000 [%]	r5000 [%]	n
Cav1.3L/β3	76.9 ± 3.3	62.2 ± 4.1	48.8 ± 4.4	22.6 ± 3.5	9
+ RIM2α	88.9 ± 2.7**	80.6 ± 3.5**	69.6 ± 4.0**	32.6 ± 4.1	11
+ RBP2	82.4 ± 2.4	70.9 ± 3.3	59.9 ± 3.8	31.8 ± 3.3	14
+ RIM2α/RBP2	94.9 ± 0.9***	90.6 ± 1.6***	83.7 ± 2.3***,#	52.0 ± 3.7***,##	17
Cav1.342A/β3	87.1 ± 2.1	76.0 ± 3.3	61.4 ± 4.2	26.1 ± 3.5	14
+ RIM2α	97.3 ± 1.0*	94.3 ± 1.6**	88.4 ± 2.1***	53.8 ± 3.6***	11
+ RBP2	88.9 ± 3.1	79.4 ± 4.4	65.9 ± 5.5	27.3 ± 2.6	9
+ RIM2α/RBP2	90.9 ± 4.3	85.1 ± 6.0	74.6 ± 7.3	45.9 ± 6.4**	9
Cav1.3L/β2a	92.0 ± 2.6	85.0 ± 3.6	77.3 ± 5.0	51.3 ± 6.7	9
+ RIM2α	91.1 ± 1.9	85.1 ± 3.2	77.7 ± 4.4	48.6 ± 6.6	7
+ RBP2	92.3 ± 0.4	85.7 ± 0.8	77.8 ± 0.9	49.5 ± 1.9	6
+ RIM2α/RBP2	92.6 ± 1.0	86.4 ± 1.7	79.3 ± 2.5	52.2 ± 3.9	10
Cav1.3L/β2e	87.9 ± 2.1	78.9 ± 3.3	65.9 ± 4.5	31.1 ± 4.0	7
+ RIM2α	90.8 ± 1.8	84.0 ± 2.5	70.0 ± 2.9	40.4 ± 3.4	8
+ RBP2	85.3 ± 4.7	77.3 ± 6.9	64.5 ± 9.1	34.8 ± 8.5	6
+ RIM2α/RBP2	87.9 ± 2.3	81.4 ± 3.1	73.0 ± 3.7	45.4 ± 3.4	10
Cav1.342A/β2a	97.6 ± 0.8	95.0 ± 1.3	89.6 ± 1.8	62.0 ± 2.8	8
+ RIM2α	96.5 ± 1.0	92.3 ± 1.6	85.6 ± 2.6	49.9 ± 3.8	10
+ RBP2	97.6 ± 0.9	94.6 ± 1.5	89.1 ± 2.2	59.9 ± 3.7	11
+ RIM2α/RBP2	96.7 ± 1.0	92.8 ± 1.5	85.8 ± 2.0	52.3 ± 2.8	10

Data are given as means ± SEM. Statistical significance was determined by one-way ANOVA with Bonferroni’s multiple comparison post hoc test as indicated in the table. Versus control (Cav1.3 without RIM2α and/or RBP2): ***p < 0.001; **p < 0.01; *p < 0.05; RIM2α versus RIM2α/RBP2 ##p < 0.01; #p < 0.05