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Effects of fluoxetine on (A) ET-1 mRNA expression (∗ P < 0.01) and (B) ECE-1 mRNA expression (∗ P < 0.01). Results were normalized to housekeeping GADPH mRNA expression. Cells were pre-incubated with the compounds for 24 h before their stimulation with TNF-α and IL-1β. RNA was isolated from the human astrocytoma cell pellets taken 6 h after their administration. Data are presented as means ± SD (n = 9).
