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. 2020 Jan 8;10:1503. doi: 10.3389/fphar.2019.01503

Figure 2.

Figure 2

Eth induces autophagy in BC cells. (A): MCF-7, MDA-MB-231, or MDA-MB-436 cells were treated with increasing concentrations of Eth for 24 h. Western blot was performed using antibodies indicated. GAPDH was used as the loading control. (B): MCF-7 (MDA-MB-231 or MDA-MB-436) cells were treated with 2.4 µM (2.0 µM or 2.0 µM) Eth for the indicated times, and cell lysates were subjected to western blot assay. (C): MCF-7, MDA-MB-231, or MDA-MB-436 cells transfected with pQCXIP-GFP-LC3 plasmid were treated with increasing concentrations of Eth for 24 h, and assessed by immunofluorescence analyses. Scale bar = 20 µm. (D): MDA-MB-231 or MDA-MB-436 cells transfected with pQCXIP-GFP-LC3 plasmid were treated with Eth (2.0 µM) and/or 3-MA (1 mM) for 24 h, and assessed by immunofluorescence analyses. Scale bar = 20 µm. (E): Graph shows quantification of LC3-positive punctate cells in (D). (F): MCF-7, MDA-MB-231, or MDA-MB-436 cells were treated with Eth (3.5 µM, 3.0 µM, or 3.0 µM) and/or 3-MA (1 mM) for 24 h and analyzed by MTT assay. (G): MCF-7, MDA-MB-231, or MDA-MB-436 cells were treated with Eth (2.4 µM, 2.0 µM, or 2.0 µM) and/or 3-MA (1 mM) for 24 h, and western blot was performed using antibodies indicated. *P < 0.05.