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. 2020 Jan 8;10:1654. doi: 10.3389/fpls.2019.01654

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Copyright © 2020 Su, Han, Min, Zhou, Zhang, Zhao and Fang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apoplastic localizations of PtC/VIF1 and 2 in tobacco and Arabidopsis. (A-F) Tobacco leaves were co-infiltrated with A. tumefaciens (C58C1) culture harboring the florescent fusion constructs of 35S: PtC/VIF1: YFP and 35S:AtCIF1: RFP or 35S: PtC/VIF2: YFP and 35S:AtCIF1: RFP. (A, D) Epidermal cells of tobacco leaf depicting YFP (green) fluorescence. (B, E) The red fluorescent signals of a cell wall marker AtCIF1. (C, F) The yellow fluorescent signals captured from the overlap of YFP and RFP fusion. (G) Images of CLSM in transgenic Arabidopsis showed the yellow fluorescent (green) signal of PtC/VIF1. Fluorescent images showing (H) YFP (green) signals, (I) the contracted vacuoles, (J) PI staining (red), and (K) the overlapping signals (yellow) from YFP and PI after plasmolysis (200 mM mannitol). PI (propidium iodide) was used as a marker to track the cell wall for fresh cells. The Arabidopsis seedlings grew for five days under short-day conditions and were harvested for the CLSM analysis.