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Polar and charged residues in position 749 result in T6SS inhibition by VgrGi. Western blot probing for Hcp expression and secretion was performed. OD-normalized whole-cell (WC) and supernatant (Sup) fractions of AbCAN2ΔvgrGi expressing the indicated VgrGi point mutants were assessed. RNAP was included as a lysis and loading control. The Western blot shown is representative of three independent experiments.
