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. 2020 Jan 14;11(1):e03103-19. doi: 10.1128/mBio.03103-19

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Copyright © 2020 Sooryanarain et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 1 — Differential induction of type I (α) and type III (λ1/3) IFN mRNA expression levels in liver tissues of conventional and gnotobiotic pigs experimentally infected with a genotype 3 human HEV. Type I (IFN-α) and type III (IFN-λ1/3) IFN mRNA expression levels in uninfected or HEV-infected conventional (N = 2 uninfected, 4 infected) (a) or gnotobiotic (N = 5 uninfected, 6 infected) (b) pigs at 4 weeks postinfection (wpi) were quantified using gene-specific RT-qPCR. Swine RSP32 was used as a housekeeping control. Fold change was calculated using the threshold cycle (2^−ΔΔ^CT) method. *, P < 0.05 versus uninfected pigs. The data represent means ± standard errors of the means (SEMs).