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. 2020 Jan 14;11(1):e03103-19. doi: 10.1128/mBio.03103-19

FIG 2.

FIG 2

HEV 3′ UTR induced higher levels of IFN mRNAs in Huh7-S10-3 liver cells. Type III IFN (hIFN-λ1) (a) and type I IFN (hIFN-β) (b) mRNA levels in plain Huh7-S10-3 cells stimulated by different HEV RNA PAMPs (SL1cap, SL1, SL2, or SL3) at 18 h poststimulation were quantified using gene-specific qPCR. The fold change was calculated compared to unstimulated cells (mock), using the 2−ΔΔCT method. RPS18 was used as a housekeeping control. (c) IFN-β promoter activity in HEV RNA PAMP-stimulated Huh7-S10-3 cells and unstimulated (mock) cells. Human IFN-β promoter firefly luciferase was used as a reporter plasmid, and TK-Renilla luciferase was used as a control vector. The IFN-β promoter activity was calculated by determining the ratio of firefly luciferase (FLuc)/Renilla luciferase (RLuc) levels as measured by Dual-Glo kit. **, P ≤ 0.01 versus HEV SL3 using paired Student t test. The data represent means ± SEMs of results from three independent experiments.