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. 2020 Jan 14;11(1):e03103-19. doi: 10.1128/mBio.03103-19

FIG 4.

FIG 4

Type I and type III IFN mRNA expression levels in HEV RNA PAMP-stimulated Tet-On RIG-I Huh7-S10-3 liver cells. Type I IFN (hIFN-β) (a) and type III IFN (hIFN-λ1) (b) mRNA expression levels in HEV RNA PAMP (SL1cap, SL1, SL2, or SL3)-stimulated Tet-On RIG-I cells at 6 h and 18 h poststimulation were quantified using gene-specific RT-qPCR. (c) RIG-I mRNA levels at 18 h poststimulation. The fold change was calculated compared to the unstimulated cells (mock) using the 2−ΔΔCT method. RPS18 was used as a housekeeping control. a, P < 0.05; aa, P < 0.01 versus HEV SL3-stimulated cells. Data represent means ± SEMs of results from two independent experiments.