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. Author manuscript; available in PMC: 2020 Jan 16.
Published in final edited form as: Oncogene. 2019 Jul 15:10.1038/s41388-019-0885-4. doi: 10.1038/s41388-019-0885-4

Figure 4: Effects of pharmacological UDP-glucuronic acid depletion in a mesenchymal-like breast cancer cell line model.

Figure 4:

A) MDA-MB-231 cells treated with 1mM 4-methylumbelliferone (4-MU) had significantly decreased intracellular UDP-GlcUA. B) MDA-MB-231 cells treated with 1mM 4-methylumbelliferone formed significantly fewer colonies in soft agar. C) Representative images from colony formation assay. D) Treatment with 1mM 4-MU significantly reduced cellular invasiveness of MDA-MB-231 cells in a hyaluronic acid (HA) -dependent manner. E) Representative images from transwell invasion assay. F) Images of excised MDA-MB-231 xenograft tumors from mice which were administered 225mg/kg 4-MU or 2% sucrose (Control) via daily oral gavage. G) Boxplots of excised tumor volumes show 4-MU treatment significant delayed tumor growth. Scale bars = 100 μm. N.S. = not significant, * p < 0.05, ** p < 0.01, *** < 0.001, versus control.