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. 2020 Jan 8;10:3032. doi: 10.3389/fimmu.2019.03032

Figure 4.

Figure 4

CD36 deficiency normalizes the resistance to subretinal elimination of Cx3cr1-deficient monocytes and reduces their IL-6 production in vitro. (A) Schematic representation of the subretinal adoptive transfer experiments. (B) Representative images and quantification of CSFE+ Mo of the indicated strains on RPE flatmounts 24 h after subretinal transfer to the subretinal space of wildtype mice (*Mann & Whitney test C57BL/6J Mo vs. Cx3cr1−/− Mo p = 0.0019; $Mann & Whitney test Cx3cr1−/− vs. Cx3cr1−/−Cd36−/− Mos p = 0.0079). (C) Quantitative RT–PCR of IL-6 mRNA normalized with S26 mRNA of C57BL/6J, Cx3cr1−/−, and Cx3cr1−/−Cd36−/− monocytes cultured for 24 h (*Mann & Whitney test C57BL/6J Mo vs. Cx3cr1−/− Mo p = 0.0159; $Mann & Whitney test Cx3cr1−/− vs. Cx3cr1−/−Cd36−/− Mos p = 0.0159). (D) IL-6 ELISA of the supernatants of C57BL/6J, Cx3cr1−/−-, and Cx3cr1−/−Cd36−/− monocytes cultured for 24 h (*Mann & Whitney test C57BL/6J Mo vs. Cx3cr1−/− Mo p = 0.0159; $Mann & Whitney test Cx3cr1−/− vs. Cx3cr1−/−Cd36−/− Mos p = 0.0079). n, indicated for each bar; Scale bar (A) = 50 μm.