FIGURE 1.

Myotube heat treatment (HT) experimental design. Myotubes were placed into one of four groups, thermoneutral (TN), 1 h of HT with no recovery (0 h), 1 h HT with 2 h of recovery (2 h), or 1 h HT with 24 h of recovery (24 h). Heating at 40°C began 1 h prior to the amount of recovery time designated for the group. Bafilomycin A1 (BafA1) was added 3 h prior to cell harvest for each group. The lines next to the group name represent time spent in 37°C, from the start of the experiment. The gray bars represent time spent in 40°C in relation to BafA1 and cell harvest. The dotted box indicates duration of BafA1 treatment.