FIGURE 2:

BH sites of Dictyostelium myosin 1s are located in conserved positions. (A) BH sites of Dictyostelium and human myosin 1s localized within the β2/β3 and β3/β4 loops of their PH domains. Note that most BH sites are located in the vicinity of loops between β1 and β2 for human (Hs) myosin 1s and between β3 and β4 for Dictyostelium (Dd) myosin 1s. Dictyostelium Myo1E and human Myo1B and Myo1D do not have BH sites in this region. The blue arrows at the bottom of the figure mark the positions of β1, β2, β3, and β4 for human Myo1C as defined from its crystal structure (Lu et al., 2015). (B) BH sites of Dictyostelium and other myosin 1s located N-terminal to the PH domain. Secondary structures are marked according to the crystal structure of HsMyo1C (Lu et al., 2015) that does not have a BH site in this region but is included in the alignment as a reference sequence. Alignments were done with MUSCLE (Edgar, 2004). BH sites were defined by BH search (Brzeska et al., 2010) and are boxed. For locations of other BH sites, see Table 1. The sequences are from Cymobase (Odronitz and Kollmar, 2006; Kollmar and Muhlhausen, 2017) and their accession names are shown in the figure. In Figure 2B, sequences from the additional following species are presented: mosquito (Aea, Aedes aegypti), bumble bee (Boi, Bombus impatiens), single-celled eukaryote (Co, Capsaspora owczarzaki), and red flour beetle (Tic, Tribolium castaneum).