TABLE 2:
Myosins and mutants used in this study.
| Protein name | Description | Mutation |
|---|---|---|
| Myo1C | Full length, 1–1182 | None |
| Myo1C-BH-Ala | BH site mutated | K838A, K845A, K847A |
| Myo1C-BH-N-Ala | BH-N site mutated | R776A, R783A, K784A |
| Myo1C-2BH-Ala | Both BH sites mutated | R776A, R783A, K784A, K838A, K845A, K847A |
| Myo1D | Full length, 1–1109 | None |
| Myo1D-BH-Ala | BH site mutated | K804A, R805A, K812A, R831A |
| Myo1E | Full length, 1–1005 | None |
| Myo1E-BH-N-Ala | BH-N site mutated | K810A, K811A, K812A |
| Myo1F | Full length, 1–1071 | None |
| Myo1F-BH-Ala | BH site mutated | K946A, K947A, K951A, K952A |
| Myo1F-BH-N-Ala | BH-N site mutated | R871A, K872A, K873A, R878A, R879A, K880A |
| Myo1B-BH-Myo1D | BH sites swapped | KKKVLVHTLIRR/RKKRPWIYVQKRR |
In Myo1B-BH-Myo1D the BH site in Myo1B (801)KKKVLVHTLIRR(812) was replaced with the BH site of Myo1D (802)RKKRPWIYVQKRR(814).