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. 2019 Nov 28;39(2):e101928. doi: 10.15252/embj.2019101928

Figure 3. Monomeric UVR8 inhibits auxin responses in a tissue‐autonomous manner.

Figure 3

  • A–C
    Phenotypic analysis. WT, GR‐UVR8R338A/uvr8, and GR‐UVR8W285F/uvr8 seedlings were grown in LD (16‐h light/8‐h dark) for 5 days, then transplanted to new medium with 0.4 μM NAA and with or without 20 μM DEX, and kept in white light plus UV‐B (1 W/m2) for 7 days. Images are shown in (A); scale bars = 2 mm. The lateral root density (number of lateral roots/length of primary root) (B) and average length of lateral roots (C) of indicated genotypes were measured. SDs (n > 8) are indicated. Letters “a” and “b” indicate statistically significant differences for the indicated values, as determined by a one‐way analysis of variance (ANOVA), followed by Tukey's least significant difference (LSD) test (P < 0.05). There was statistically significant difference between values marked with different letters (for example “a” and “b”), while there was no statistically significant difference between values marked with the same letters (for example “a” and “a”).
  • D–F
    UVR8 inhibited auxin responses under UV‐B in a tissue‐autonomous way. WT and uvr8 seedlings grown in LD for 5 days were used for reciprocal grafting. Seedlings were kept in LD for 7 days after grafting, then transplanted to new medium containing 0.4 μM NAA, and kept in UV‐B light (1 W/m2) for 10 days. Images are shown in (D); scale bar = 2 mm. The lateral root density (number of lateral roots/length of primary root) (E) and average length of lateral roots (F) of indicated genotypes were measured. SDs (n > 8) are indicated. Letters “a” and “b” indicate statistically significant differences for the indicated values, as determined by a one‐way analysis of variance (ANOVA), followed by Tukey's least significant difference (LSD) test (P < 0.05). There was statistically significant difference between values marked with different letters (for example “a” and “b”), while there was no statistically significant difference between values marked with the same letters (for example “a” and “a”).