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. 2019 Nov 28;39(2):e101928. doi: 10.15252/embj.2019101928

Figure EV4. UVR8 physically interacts with MYB73/MYB77 in a UV‐B‐enhanced manner.

Figure EV4

  1. β‐gal assay showing the UV‐B‐dependent interaction between UVR8 and MYB73. β‐gal activity was present in yeast cells expressing UVR8 and MYB73 together in the presence of UV‐B and almost absent in yeast cells in the absence of UV‐B or expressing MYB73 alone. Error bars, SDs of three biological replicates.
  2. BiLC assay showing that UV‐B treatment promotes the formation of the UVR8–MYB73 complex. Leaf epidermal cells of N. benthamiana were co‐transformed with MYB73‐nLUC and cLUC‐UVR8 and treated with or without UV‐B (2 W/m2) for 30 min before being photographed. Scale bar = 1 cm.
  3. Quantification of luciferase (LUC) activities of MYB73‐nLUC + cLUC‐UVR8 in (B) with or without UV‐B treatment (UV‐B + white light versus white light only). Error bars, SDs of three biological replicates.
  4. BiLC assay showing that UV‐B treatment promotes the formation of the UVR8–MYB77 complex. Scale bar = 1 cm.
  5. Quantification of LUC activities of MYB77‐nLUC + cLUC‐UVR8 in (D) with or without UV‐B treatment. Error bars, SDs of three biological replicates.