Figure EV5. MYB73/MYB77 works redundantly to mediate UVR8‐controlled auxin responses.
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AqPCR assay showing that MYB73/MYB77 is expressed in both the shoot and root. Wild‐type seedlings were grown in 1/2 MS for 12 days. ACT7 expression was analyzed as an internal control. Error bars are SD of three biological replicates.
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BPhenotypic analysis. Seedlings of the indicated genotypes were grown in 1/2 MS with UV‐B treatment for 2 weeks. Scale bars = 2 mm.
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C, DPhenotypic analysis. Seedlings of the indicated genotypes were grown in LD (16‐h light/8‐h dark) conditions for 5 days and then transplanted to new plates containing 0.4 μM NAA with UV‐B treatment for 7 days. Images are shown in (C); scale bars = 2 mm. The lateral root density (number of lateral roots/length of primary root) and average length of lateral roots of the indicated genotypes were measured (D). SDs (n > 8) are indicated. Letters “a” and “b” indicate statistically significant differences for the indicated values, as determined by analysis of variance (ANOVA), followed by Tukey's least significant difference (LSD) test (P < 0.05). There was statistically significant difference between values marked with different letters (for example “a” and “b”), while there was no statistically significant difference between values marked with the same letters (for example “a” and “a”).