Figure 7. UVR8 inhibits the DNA‐binding activity of MYB73/MYB77.

• A
  Electrophoretic mobility‐shift assay (EMSA) showing that MYB73/MYB77 binds to the MBSI motif in vitro. Cold probe was added as a competitor.
• B
  EMSA showing that MYB73/MYB77 binds to the promoter of IAA19. Cold IAA19p or IAA19mp (MBSI motif mutated) was added as a competitor.
• C, D
  EMSA results showing that monomeric UVR8 inhibits the DNA‐binding activity of MYB73(C) and MYB77(D).
• E
  ChIP‐qPCR analysis showed that UV‐B inhibits the DNA‐binding activity of MYB73. ChIP‐qPCR assays were performed using transgenic seedlings expressing 35S::MYB73‐TAP, treated with or without UV‐B for 5 h before harvesting samples. Chromatin fragments (˜500 bp) were immunoprecipitated by anti‐Myc antibody, and the precipitated DNA was analyzed by qPCR using the primer pairs indicated. The IP/Input ratios are shown with SDs (n = 3).
• F
  ChIP‐qPCR showing that UV‐B inhibits the DNA‐binding activity of MYB73 in a UVR8‐dependent manner. The IP/Input ratios are shown with SDs (n = 3).
• G, H
  ChIP‐qPCR showing that UV‐B inhibits the DNA‐binding activity of MYB73 in roots (G) in a UVR8‐dependent manner (H). The IP/Input ratios are shown with SDs (n = 3).
• I
  ChIP‐qPCR showing that UV‐B inhibits MYB77 from binding to the indicated promoters in a UVR8‐dependent manner. The IP/Input ratios are shown with SDs (n = 3).

Source data are available online for this figure.