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. 2019 Dec 5;39(2):e102602. doi: 10.15252/embj.2019102602

Figure 3. GMV‐triggered plant hyper‐sensitivity to Pi deficiency is mediated by diacetyl.

Figure 3

  • A
    Images of Arabidopsis plants exposed to four individual GMV components, including diacetyl (DA), 2,3‐butanediol (BTDL), 2‐methyl‐1‐propanol (MP), and acetoin (ATN). The compounds were applied at dosages that, when the compounds totally evaporate from the agar‐containing solid droplets, would yield in volatile concentrations of 9.7 μg (DA), 32.5 μg (BTDL), 7.9 μg (MP), and 28.5 μg (ATN) per ml free space in the petri dish, which resembled the 1:3.3:0.8:2.9 ratio among the four GMV components in natural GMVs.
  • B, C
    Anthocyanin hyper‐accumulation (B) and PSR gene hyper‐induction (C) were observed in Pi‐deficient plants exposed to DA but not to the other three GMV components.
  • D, E
    Compared with the wild‐type plants, phr1phl1 showed substantially decreased anthocyanin hyper‐accumulation (D) and PSR gene hyper‐induction (E) triggered by DA.
Data information: The boxplots show representative data from three independent experiments (n = 6). Whiskers represent the min to max data range, and the median is represented by the central horizontal line. The upper and lower limits of the box outline represent the first and third quartiles. qPCR results show values of means ± SE of two biological replicates. Different letters denote significantly different means at P < 0.05, Tukey's multiple comparison test.