Figure EV3. Deleterious effects caused by DA are due to an activation of immunity in Pi‐deficient plants (related to Fig 4).

• A
  DA induces genes involved in JA biosynthesis and signaling in Arabidopsis grown with Pi deficiency.
• B
  DA elevates JA accumulation levels in Pi‐deficient Arabidopsis. Means ± SE of three biological replicates.
• C
  Images of plants grown with different treatments using DA (9.7 μg/ml free space) or MeJA (25 μM in plant growth medium).
• D, E
  Exogenous application of MeJA mimics DA‐induced anthocyanin accumulation (D) and PS2 gene induction (E) patterns in Pi‐deficient plants.
• F, G
  Compared with the wild‐type plants, the JA‐insensitive coi1 mutant plants showed altered responses to DA under Pi‐deficiency condition, as shown by plant images (F) and quantification of anthocyanin accumulation levels (G).

Data information: The boxplots show representative data from three independent experiments (n = 6). Whiskers represent the min to max data range, and the median is represented by the central horizontal line. The upper and lower limits of the box outline represent the first and third quartiles. qPCR results show values of means ± SE (n = 3), and two biological replicates were analyzed with similar results. Different letters denote significantly different means at P < 0.05, Tukey's multiple comparison test.