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Schematic illustration of the living lactam biosensors. (a) The ChnR/Pb transcription factor-promoter pair-based lactam sensing pathway engineered in E. coli. (b) The hydrogel-based living biosensors generated by encapsulating engineered E. coli. cells into agarose microgels. The recovered microgels are further incubated to form colonies and express mCherry fluorescent protein if lactam is present in the environment, which can be fluorescently detected.
