Table 1.
Reference | Process | Solvent | T (°C) | Time (min) |
---|---|---|---|---|
D1 | Autoclave | MilliQ water | 121 | 30 |
D2 | Short Alkaline Boiling | Na2CO3 0.02 mol/L | 100 | 30 |
D3 | Intensive Alkaline Boiling | Na2CO3 0.02 mol/L | 100 | 120 |
D4 | Ultrasonication with probe * | MilliQ water | 60 | 60 |
* Branson Sonifier SFX-550 (Emmerson Ultrasonic Corporation. Dansbury, CT, USA) equipped with a disruptor horn and a 1/8” diameter tapered microtip. Sonication was set for 30 min with 50% amplitude, and the maximum temperature was set at 60 °C.