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. 2019 Dec 9;19(24):5409. doi: 10.3390/s19245409

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The binding preference of the Tn glycan biosensor was investigated using a negative control (Con A, a lectin not recognizing Tn antigen), a positive control (DBA lectin, recognizing Tn antigen) and an analyte (a tumor associated antibody 2C4). DPV was measured in an electrolyte containing 5 mM potassium hexacyanoferrate (II) trihydrate and 0.01 M PBS, pH 7.4. The parameters applied for the differential pulse voltammetry were as follows: 60 s accumulation time at 0.2 V, 50 ms modulation time, 0.5 s interval time, 25 mV modulation amplitude, and 5 mV step. Linear regression fit for DBA: ΔI = (−55.6 ± 4.8) + (−2.82 ± 0.34)×c, R² = 0.918 and for the 2C4 antibody ΔI = (−28.3 ± 2.0) + (−1.37 ± 0.14)×c, R² = 0.940. The plot for Con A could not be linearly fitted.