Fig. 2.
Force-pCa curves of pediatric skinned cardiomyocytes. A: relative force generation at given pCa, demonstrating a leftward shift in dilated cardiomyopathy (DCM) cardiomyocytes compared with nonfailing (NF) cardiomyocytes indicative of increased calcium sensitivity. B: there is normalization of calcium sensitivity in DCM cardiomyocytes with in vitro protein kinase A (PKA) treatment (rightward shift). C: although there is a subtle rightward shift of the relative force-pCa curve with in vitro PKA treatment of NF cardiomyocytes, there is no statistically significant difference in pCa50. D: absolute force development over a range of calcium concentrations (pCa). NF: no. of subjects (N) = 8, no. of cardiomyocytes (n) = 33; NF+PKA: N = 8, n = 29; DCM: N = 8, n = 26; DCM+PKA: N = 8, n = 26.