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. 2019 Nov 15;2(4):86. doi: 10.3390/mps2040086

Table 4.

Comparison of H&E re-used and sequential comparator immunostaining intensity scores.

Marker Re-used H&E Stain Intensity Sequential Stain Intensity: 0–3 (int ctrl) Initial H&E Assessment
HMWCK + p63 3 2 2 2 0 3 atrophy inflammation
CK8&18 3 3 3 3 3 3 Atrophy + inflammation: whole glands
CD49f 3 (2) 3 (1) 3 (1) 3 (1) 3 (1) 3 (2) no cancer, int ctrls
E-cadherin 3 3 3 3 3 3 not much cancer but weird well differentiated
ERG 3 (3) 0 (3) 0 (3) 0 (0) 1 (3) 0 (3) lots of infiltrating lymphocytes, grade 3 + 3 fragmented tumor lost basal cells, int ctrls

Abbreviation: int ctrls, internal controls.