Table 3.
Effect of cytokinins on shoot regeneration from leaf and node derived callus cultures in Morus alba L. cv. Chinese white.
| Plant Growth Regulators (μM/L) |
No. of shoots regenerated after 4 weeks of Culture(X*± S.E) |
||||
|---|---|---|---|---|---|
| BAP | Kn | TDZ | BAP + TDZ | From Leaf Callus | From Nodal Callus |
| 2.5 | – | – | – | 0.6 ± 0.10j | 0.00 ± 0.00f |
| 5.0 | – | – | – | 2.1 ± 0.34h | 0.00 ± 0.00f |
| 7.5 | – | – | – | 2.4 ± 0.59h | 1.8 ± 0.16e |
| 10.0 | – | – | – | 1.4 ± 0.32i | 1.6 ± 0.38e |
| 12.5 | – | – | – | 1.6 ± 0.18i | 3.2 ± 0.64d |
| 15.0 | – | – | – | 1.0 ± 0.12i | 1.0 ± 0.08e |
| – | 2.5 | – | – | 0.00 ± 0.00k | 0.00 ± 0.00f |
| – | 5.0 | – | – | 1.4 ± 0.16i | 1.2 ± 0.10e |
| – | 7.5 | – | – | 1.2 ± 0.24i | 1.6 ± 0.28e |
| – | 10.0 | – | – | 3.8 ± 0.42c | 3.0 ± 0.48d |
| – | 12.5 | – | – | 2.6 ± 0.34h | 2.4 ± 0.22e |
| – | 15.0 | – | – | 2.2 ± 0.62h | 2.1 ± 0.36e |
| – | – | 1.0 | – | 2.2 ± 0.52h | 2.0 ± 0.18e |
| – | – | 1.5 | – | 3.4 ± 0.32g | 3.2 ± 0.42d |
| – | – | 2.0 | – | 5.8 ± 0.44e | 4.6 ± 0.32c |
| – | – | 2.5 | – | 3.6 ± 0.56g | 3.2 ± 0.55d |
| – | – | 3.0 | – | 1.8 ± 0.38i | 2.4 ± 0.40e |
| – | – | 5.0 | – | 1.4 ± 0.24i | 1.2 ± 0.32f |
| – | – | – | 2.5 + 2.0 | 12.2 ± 0.68a | 3.2 ± 0.67d |
| – | – | – | 5.0 + 2.0 | 10.4 ± 0.56b | 4.2 ± 0.52c |
| – | – | – | 7.5 + 2.0 | 08.2 ± 0.46d | 8.6 ± 0.72a |
| – | – | – | 10.0 + 2.0 | 07.4 ± 0.82c | 5.8 ± 0.58b |
| – | – | – | 12.5 + 2.0 | 04.6 ± 0.67f | 3.7 ± 0.36d |
| – | – | – | 15.0 + 2.0 | 02.4 ± 0.38h | 2.8 ± 0.42e |
Medium: MS medium with 3 % sucrose & 0.8 % agar supplemented with different conc. of BAP, Kn and TDZ.
Data represent the mean + SE of three randomly selected readings of ten replicates per treatment at the end of 4 weeks of culture. Bold characters denote significant response and values. Within columns, means followed by the same letter did not differ significantly according to Tukey’s test of SPSS Version 17 at P = 0.05.