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. 2019 Oct 26;2(4):83. doi: 10.3390/mps2040083

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Workflow showing toolkit for phenotyping hypertrophic cardiomyopathy (HCM) in cellular models. Cardiomyocytes can be obtained from different sources (biopsies or cell lines) and require dissociation into a single cell suspension prior to phenotyping. Evaluation of in vitro hallmarks of HCM is done via: (1) Flow volumetry to investigate hypertrophy; (2) high-content imaging to assess multinucleation, brain natriuretic peptide (BNP) hypertrophic marker expression, and sarcomeric disarray; (3) Seahorse assay and qPCR to assess mitochondrial respiration and content, respectively.