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. 2019 Oct 26;2(4):83. doi: 10.3390/mps2040083

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of mitochondrial respiration using Seahorse platform. (A) Protocol summary for mitostress test using the Seahorse assay™. (B) Representative micrographs detailing normalisation by automated quantification of cell nuclei in Cellavista™ plate imager ((i) Hoechst staining of nuclei; (ii) quantification of individual nuclei; (iii) overlay). (C) Sample graph for oxygen consumption rate (OCR) over time following the Seahorse mitostress kit, highlighting the different mitochondrial respiration parameters.