Table 1.

Surface areas and required cell numbers and volumes for culturing primary HUFs (48 h).

Dish	T25	T75	T175	6-Well	96-Well
Surface area (cm2)	25	75	175	9.6	0.32
Cells/dish (× 106)	0.4	1.2	2.8	0.15	0.005
Medium for culture (mL)	5	12	35	2	0.1
PBS for washing (mL)	5	10	12	2	-
Trypsin for harvesting (mL)	1	2	3	0.5	-
Medium to inactivate trypsin (mL)	4	8	17	1	-