Table 2.

Splitting ratios for the HIMA.

Protocol Stage	Day	Passage	Ratio
Set-up	6	1	150,000 cells/well
Pre-senescence	9–10	2	1:3–1:5
	11–17	3–4	1:2.5–1:4
	19–24	5	1:1.5–1:3
Senescence	18+	5+	1:1
Clones emerging	32+	6+	1:1.5–1:2.5
Nutlin-3a counter-screen	32+	7+	1:3–1:50
Expansion into cell lines	32+	8+	1:3–1:50