Table 3.

Preparation of lysis buffer.

	Concentration	Final Concentration	Volume (mL)
Tris pH 6.8	1000 mM	62.5 mM	1.25
EDTA pH 8.0	500 mM	1 mM	0.04
SDS	10%	2%	4
Sterile glycerol	40%	10%	5
Water	-	-	9.75
Inhibitors	100X	1X	see text