Table 5.

Preparation of primary antibody solutions.

Antibody	Dilution	3% Milk in TBST (mL)	Primary Antibody (µL)	Sodium Azide (µL)
p53	1:500	10	20	100
p-p53	1:2000	10	5	100
p21	1:2000	10	5	100
pChk1	1:1000	10	10	100
pH2ax	1:1000	10	10	100
Mdm2	1:750	10	13.3	100
Gapdh	1:25,000	40	1.6	400