Table 7.
Primer information for PCR amplification and expected product size.
| Exon | Primer Name | Forward/ Reserve |
Primer Sequence (5′ to 3′) | Product Size (bp) |
|---|---|---|---|---|
| 4 | 4F | Forward | GTC CTC TGA CTG CTC TTT TCA CCC ATC TAC | 366 |
| 4R | Reverse | GGG ATA CGG CCA GGC ATT GAA GTC TC | ||
| 5 & 6 | 5F | Forward | CTT GTG CCC TGA CTT TCA ACT CTG TCT C | 478 |
| 6R | Reverse | GCC ACT GAC AAC CAC CCT TAA CCC CTC | ||
| 7 | 7F | Forward | AGA TCA CGC CAC TGC ACT C | 376 |
| 7R | Reverse | CCG GAA ATG TGA TGA GAG GT | ||
| 8 & 9 | 8_9F | Forward | CAA GGG TGG TTG GGA GTA GA | 498 |
| 8_9R | Reverse | GTC TCT GGC ATG CGA CTC TC |