Table 8.

Preparation of PCR Master Mix.

PCR Reaction Component	Concentration	Final Concentration	Reaction Volume (µL)
REDTaq®		1X	12.5
Forward primer	10 µM	20 pmol	2
Reverse primer	10 µM	20 pmol	2
DNA	~100 ng/µL	200 ng	2
Nuclease-free water	‒	‒	6.5
Total volume	‒	‒	25