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. 2020 Jan 14;27(1):1073274819897977. doi: 10.1177/1073274819897977

Figure 2.

Figure 2.

Effect of overexpressing lncRNA NEAT1 in proliferation and apoptosis of glioma cells. A, Glioma cell lines (U-87 MG and U251) were transiently transfected with the NEAT1-overexpressing vector (pcDNA-NEAT1), and the upregulation of NEAT1 after the transfection was confirmed using qRT-PCR. B, The cell proliferation after overexpressing NEAT1 was determined using MTT assay. C, The cell apoptosis after overexpressing NEAT1 was determined using flow cytometry. *P < .05, **P < .01 vs pcDNA. lncRNA indicates long noncoding RNA; MTT, 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide; NEAT1, nuclear-enriched abundant transcript 1; pcDNA, the negative control of pcDNA-NEAT1; qRT-PCR, quantitative real-time polymerase chain reaction.