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. 2020 Jan 14;27(1):1073274819897977. doi: 10.1177/1073274819897977

Figure 4.

Figure 4.

Nuclear-enriched abundant transcript 1 modulates DKK3 expression via regulating miR-92b. The glioma cell lines (U-87 MG and U251) were transfected with pcDNA-NEAT1 or cotransfected with miR-92b mimic. The (A) mRNA and (B) protein expressions of DKK3 after transfection were determined using qRT-PCR and Western blot analysis, respectively. Prenegative control (Pre-NC) was used as the control of miR-92b mimic. The glioma cell lines (U-87 MG and U251) were also transfected with shNEAT1 or cotransfected with miR-92b inhibitor. The (C) mRNA and (D) protein expressions of DKK3 after transfection were determined using qRT-PCR and Western blot analysis, respectively. Negative control (NC) was used as the control of miR-92b inhibitor. **P < .01 vs pcDNA or shRNA. ## P < .01 vs pcDNA-NEAT1 + pre-NC or shNEAT1 + NC. mRNA indicates messenger RNA; NEAT1, nuclear-enriched abundant transcript 1; shRNA, short hairpin RNA; qRT-PCR, quantitative real-time polymerase chain reaction.