Fig. 4.

Initial Seam Assignment and X/Y Shift Smoothing. a) MT-Rot angle assignment for a single MT from the CKK dataset, after the ‘Global Search’ step, with the MT Rot angle plotted as a function of the particle number within that MT. The rainbow coloured lines show the clusters calculated during the MT-Rot angle assignment step, with each cluster representing a different PF register being aligned between individual MT particles and the reference – as can be seen by the regular spacing between the clusters. A MT top view representation is annotated by the percentage of particles for this MT aligned with different PFs in the 3D reference. b) Rot angles from the MT example in a), after MT Rot angle assignment. c) The X/Y shifts from the example MT in a-b plotted as a function of particle number. The micrograph of the MT is shown, with the X/Y alignment for each particle in the MT represented by green circles. Particles which have shifted in register are shown by black arrows. d) The X and Y shifts for the example MT in a-c, after X/Y shift smoothing.