Table 1.
Dataset and data collection details. *K2 summit direct electron detector from Gatan Inc. CA, USA. †DE20 direct electron detector from Direct Electron, San Diego, CA. ‡With quantum post-column energy-filter (Gatan Inc. CA, USA), operated in zero-loss imaging mode with a 20-eV energy-selecting slit. §C-Flat 2/2-4C from Protochips Inc. ‖Lacey carbon grids from Agar Scientific.
| Decorator | Tubulin Ligands | Grid type | EM and energy filtration | Detector and mode | Pixel size (Å/pixel) | Defocus range | Dose (e-/Å2, weighted) | Total exposure time and frame number | EMPIAR and EMDB accession codes |
|---|---|---|---|---|---|---|---|---|---|
| CKK domain of CAMSAP1 | α-tubulin: GTP β-tubulin: GDP, Taxol |
2 μm Holey Carbon§ | Polara (300 kV‡) | K2 summit* Counting at 5e-/pixel/sec | 1.39 | 0.5–3.5 μm | 42 dose weighted | 16 secs 64 frames | EMPIAR-465 EMD-4643 |
| Motor domain of MKLP2 | α-tubulin: GTP β-tubulin: GDP, Taxol |
2 μm Holey Carbon§ | Polara (300 kV) | DE20† (Direct Electron) Linear |
1.53 | 0.5–3.0 μm | 50 dose weighted | 1.5 secs 22 frames |
EMPIAR-467 EMD-10131 |
| N-DC domain of DCX | α-tubulin: GTP β-tubulin: GDP |
Lacey Carbon‖ | Polara (300 kV‡) | K2 summit* Counting at 5e-/pixel/sec |
1.39 | 0.5–2.5 μm | 24 unweighted | 9 secs 36 frames |
EMPIAR-10300 EMD-10195 |