Fig. 2.

Assessment of DFP-induced mitophagy in SH-SY5Y and ARPE-19 cells. (A/B) Representative widefield fluorescence images of SH-SY5Y and ARPE-19 cells expressing the mito-QC reporter. Mitophagy was stimulated with 1 mM DFP for 24 h and the number of mitolysosomes was quantified from three independent experiments with >50 cells for experiment. Scale bar = 10 μm. (C) Total number of mitolysosomes counted per cell. (D) Total number of mitolysosomes counted per cell area defined by the manual selection of ROIs. (E) Mitolysosomal area expressed in percent of the cell area. (F) Mean size of the mitolysosomes. Quantification was performed with the following parameters: Radius for smoothing images = 1; Ratio threshold = 0.6; Red channel threshold = Mean + 0 stdDev. Statistics were realised using unpaired Student’s t-test n = 3 independent experiments. Statistical significance is displayed as **p < 0.01, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).