Fig. 4.

The red-only puncta quantified by the mito-QC Counter are mitolysosomes. Representative confocal images of SH-SY5Y cells expressing the mito-QC reporter and stained with anti-LAMP1 antibody. Mitophagy was induced with 1 mM DFP for 24 h. Zoom insets show representative mitolysosomes (red-only dots) co-localising with LAMP1 structures. White arrows highlight example of quantified mitolysosomes. Scale bar = 10 μm. Quantification was performed with the following parameters: Radius for smoothing images = 2; Ratio threshold = 1.5; Red channel threshold = Mean + 0.5 stdDev. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).