Table 1.
Measurements made and parameters used by the mito-QC Counter.
| Column title | Measurement |
|---|---|
| Image | Analysed image name. |
| CellROI | Region of interest within the image. |
| CellArea_μm2 | Area of the ROI. |
| greenMean | Mean of green intensity within the ROI. This value can be extrapolated as a reading for mitochondrial content. |
| redMean | Mean of red intensity within the ROI. |
| RGratio | = redTotal/greenTotal |
| nMitolysosomes | Number of mitolysosomes detected within each ROI/cell. |
| nML_per_um2 | Mitolysosomes/cell area = nMitolysosomes/CellArea_μm2 |
| nML_over_green | Mitolysosome/mitochondrial content = nMitolysosomes/greenMean |
| MLtotalArea_μm2 | Total area of the mitolysosomes within the ROI. |
| MLpctArea | Percentage of the cell occupied by mitolysosomes. = (mitoArea_μm2/ CellArea_μm2)*100 |
| MLmeanArea_μm2 | Mean mitolysosome size = mitoArea_μm2/ nMitolysosomes |
| MLarea_μm2_over_green | Mitolysosome area/mitochondrial content = MLtotalArea_μm2/greenMean |
| X_tl | X coordinate of the ROI bounding rectangle top left corner. |
| Y_tl | Y coordinate of the ROI bounding rectangle top left corner. |
| ratioThresh | Threshold used for detecting the peaks of intensity in the ratio channel. User-defined in the settings of the mito-QC Counter. |
| redThresh | Threshold used for the red channel. User-defined in the settings of the mito-QC Counter. By default, this value is the mean red intensity of the image. |
| smoothRad | Radius for smoothing. User-defined in the settings of the mito-QC Counter. |