. 2020 Jan 14;13:28. doi: 10.1186/s13071-020-3899-4

Table 2.

Primers for amplification and sequencing

Gene	Primer	Sequence (5′–3′)	Annealing T (°C)
16S rDNA	16SF	CGGGGGAAAAATTTATTGCT	55
16S rDNA	16SR	AGCTGTAATACAGAAAGTAAA	55
wAlbA-wsp	WAF	CCAGCAGATACTATTGCG	55
wAlbA-wsp	WAR	AAAAATTAAACGCTACTCCA	55
wAlbB-wsp	WBF	AAGGAACCGAAGTTCATG	55
wAlbB-wsp	WBR	AAAAATTAAACGCTACTCCA	55
wsp	81	TGGTCCAATAAGTGATGAAGAAAC	53
wsp	691	AAAAATTAAACGCTACTCCA	53
FtsZ	ftsZ-F	TACTGACTGTTGGAGTTGTAACTAAGCCGT	58
FtsZ	ftsZ-R	TGCCAGTTGCAAGAACAGAAACTCTAACTC	58
28S rRNA	28F	TACCGTGAGGGAAAGTTGAAA	55
28S rRNA	28R	AGACTCCTTGGTCCGTGTTT	55
cox1	cox1F	TTTACAATTTATCGCCTAAACTTC	55
	cox1R	CATTGCACTAATCTGCCATA	55
	cox1f	GGGGGAGACCCTATTTTATA	55
	cox1r	TAAACTTCAGGGTGACCAAAAAATCA	55
wAlbAq-wsp	qAF	GGGTTGATGTTGAAGGAG	55
wAlbAq-wsp	qAR	CACCAGCTTTTACTTGACC	55
wAlbBq-wsp	qBF	ACGTTGGTGGTGCAACATTTG	58
wAlbBq-wsp	qBR	TAACGAGCACCAGCATAAAGC	58
RPS	RPS6-F	CGTCGTCAGGAACGTATTCG	55
RPS	RPS6-R	TCTTGGCAGCCTTGACAGC	55

Note: Primers cox1f/cox1r were used for sequencing

Abbreviation: T, temperature