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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Mol Biol. 2019 Nov 2;432(6):1801–1815. doi: 10.1016/j.jmb.2019.10.021

Figure 2: Overview of methods that directly quantify TF binding preferences to methylated DNA in vitro.

Figure 2:

Four main assay types are shown, each highlighting important features: Left: EpiSELEX-seq and methyl-Spec-seq both infer quantitative 5mC effect sizes from mixed (barcoded) pools of methylated and unmethylated DNA ligands sequenced after one round of TF binding enrichment. Right: Methyl-HT-SELEX and methyl-PBM estimate 5mC effect sizes by comparing TF binding enrichment scores between unmethylated or methylated DNA ligands. For a detailed description see Table 1.