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. 2020 Apr 29;4:150. Originally published 2019 Oct 8. [Version 2] doi: 10.12688/wellcomeopenres.15447.2

Figure 4. NEUROD1-KO cells demonstrate elevated ER stress and apoptosis.

Figure 4.

( A) PCR product for lentiCRISPRv2 and NeuroD1 sgRNA in control EV and NEUROD1-KO cells as described in Figure 2A. ( B) Western Blot analysis for NEUROD1 expression in EV and NEUROD1-KO cells one week apart (w1 and w2). ( C– F) Western blot analysis in EV and NEUROD1-KO (w1) for pPERK ( C), pIRE1 ( D), CHOP ( E) and cleaved caspase 3 ( F). GAPDH or β-Tubulin are indicated as loading controls and data is from one NEUROD1 KO cell line. Values below each lane represent the fold change of protein levels in NEUROD1-KO cells compared to EV cells.