Figure 2. CbcAgo is active in DNA-DNA interference assays.

The wild-type (Wt) and inactive DE mutant (DE) of the CbcAgo protein were pre-incubated with the indicated 5’ phosphorylated guide DNA for 10 min at 37 °C and further used to cut a complementary 45-nucleotide ssDNA target at the same temperature for 1 h. The reactions were carried out in the presence of 2 or 4 mM MnCl ₂; target (T), guide (G), and the major 34-mer product (P) of the reaction were identified in an 18% U-PAGE gel.