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Cloning mRNA. (A) Partial digestion of RNA using nuclease P1. M, a total RNA size marker with the estimated sizes using 5.8S rRNA (∼160 nt), 5S rRNA (∼120 nt), and tRNA (∼80 nt); a twofold serial titration of substrate total RNA from 2000 to 8 ng was partially digested with nuclease P1; “-” is the negative control using ∼16 ng purified mRNAs incubated without P1. (B) Fragmented mRNA was cloned as a cDNA library and resolved on an 8% native PAGE gel, as compared to the size marker M.
