Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan;30(1):127–137. doi: 10.1101/gr.248286.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Barrio-Hernandez et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 1. — Quantitative mass spectrometry revealed widespread changes during the initial period of osteoblast commitment. (A) Flow diagram of the quantitative proteomic and phosphoproteomic experiment. Human MSCs were labeled with the indicated combinations of arginine (Arg) and lysine (Lys) SILAC amino acids, induced to undergo osteoblast differentiation, and harvested at indicated time points. After cell lysis and fractionation, proteins were pooled, digested, and either analyzed by LC-MS/MS or enriched for phosphorylated species followed by LC-MS/MS analysis. (B) Numbers of significantly changed phosphorylation sites (left) and proteins (right) at indicated time points during osteoblastogenesis relative to undifferentiated cells.