Figure 10.

Maize-T. virens Interactions Upregulates Genes for 12-OPDA Biosynthesis and Response, but Not Genes Downstream of 12-OPDA for JA or JA-Ile Biosynthesis and Signaling.

(A) to (D) Heatmaps of transcript accumulation measured by RNA-seq of (A) the AOS pathway branch genes, (B) 12-OPDA–responsive genes, (C) JA downstream signaling genes, and (D) SA biosynthesis and response genes, represented by Z-score–transformed FPKM (number of standard deviations above/below the mean of the row). Values represent average FPKM, with asterisks (*) denoting significant differences compared with untreated plants (Tukey’s HSD test, P < 0.05). Treatments comprised of untreated B73 plants (n = 4), and TvWT-treated plants (n = 10) at 6 and 54 h. Gene IDs and functions of each gene are listed in Supplemental Table 2. n is the number of biological replicates consisting of plants grown in hydroponic jars, each consisting of five maize seedlings.