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. 2019 Nov 15;32(1):206–225. doi: 10.1105/tpc.19.00226

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Figure 7. — EMSAs and Yeast One-Hybrid Analysis.

(A) EMSAs showing the binding of AtZP1 to the A[AG/CT]CNAC regions of bHLH gene promoters. glutathione S-transferase–tagged AtZP1 and biotinylated A[AG/CT]CNAC probe were used. The competitor was nonbiotinylated A[AG/CT]CNAC at 10-, 100-, and 200-fold the amount of biotinylated probe. WT, wild type.

(B) Interaction of AtZP1 with the bHLH gene promoters verified by yeast one-hybrid analysis. Detection of LacZ activity in cotransformed yeast with different expression vectors pB42AD-ZP1 and pLacZi-A[AG/CT]CNAC. Three cotransformation combinations, pB42AD and pLacZi, pB42AD and pLacZi-A[AG/CT]CNAC, and pB42AD-ZP1 and pLacZi, were used as the controls.